Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandis

Heosemys grandis , a species of Asian water turtle, that has wide range applications in the food and pharmaceutical industries. Since some processed products cannot be identified exclusively by morphological microscopic identification, reliable quick approach to guarantee authenticity is critical. Thus, we fostered an effective stable molecular identification system identify based on DNA mini-barcoding species-specific primers PCR technology. A total 48 turtle samples from 16 different were collected. To distinguish its counterfeits, pair designed verified after analyzing COI sequences samples. The results showed only could generate single clear band following amplification using primers. Employing amplify can verify sequence alignment. These findings indicated technology combined with accurately detect . This broadens application biology techniques It provides convenient method for identifying raw materials standardize market protect customers’ rights interests.